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Young-Hoon Joo

Young-Hoon Joo

Catholic University of Korea, Republic of Korea

Title: Head and Neck Squamous Cancer Stem Cells and Metastasis in vitro and in vivo in the Chick Chorioallantoic Membrane Assay

Biography

Biography: Young-Hoon Joo

Abstract

Background：To evaluate the impact of CSC proportion on metastasis in head and neck
squamous cell carcinoma (HNSCC) using in vitro and in vivo models.

Method: In vitro motility was assessed using wound healing and matrigel invasion assays. Tumorigenesis and metastasis were assessed in a chick chorioallantoic membrane (CAM) assay.

Results: Flow cytometry demonstrated 60-70% CD44+ cells in UMSCC-110 and 30-40% in UMSCC-106.  UMSCC-110 cells demonstrated significantly faster wound closure (p<0.05) and more efficient invasion (p=0.033) compared to UMSCC-106 cells. In the CAM assay, there was a significant difference in liver metastasis between CD44high cells and unsorted or CD44low cells (p=0.027 in UMSCC-110 and p=0.035 in UMSCC-106, respectively).

Conclusion: The proportion of CSC was significantly related to mobility and metastasis in HNSCC cell lines. This concept might help to improve understanding of mechanisms of metastasis and change therapeutic approaches to treating cancer metastasis.

Keywords: Squamous Cell Carcinoma; Head and Neck Neoplasms; Neoplastic Stem Cells; Neoplasm Metastasis; Cell Line