10^th Global Summit on Immunology and Cell biology May 11-12, 2018 Osaka, Japan

Biography:

Zhi-Zhang Yang is an assistant professor at Mayo Clinic. He has published more than 30 papers in reputed journals such as Journal of Clinical Investigation, Blood, Cancer Research and Leukemia. He has been serving as reviewers for journals and funding grants.

 

Abstract:

T cells in the tumor microenvironment of follicular lymphoma (FL) are heterogeneous in phenotype and different subsets have differing impact on patient outcome. Using mass cytometry (CyTOF), we identified at least 12 subsets of CD4+ T cells in FL biopsy specimens, and found that some subsets were more prevalent in FL and less prevalent in tonsil tissue. Specifically, we found that CD4+ T cells in FL more frequently had a memory phenotype, but that the number of naïve T cells, rather than memory cells, was associated with a favorable clinical outcome. To determine which memory T cell populations may negatively affect prognosis, we focused on 6 subsets of memory cells, two populations that express CD25 and four that express PD-1. In FL, one of the subsets of CD4+CD25+ T cells had decreased expression of CD27 and CD28 and this subpopulation was expanded when compared to controls. Similarly, in the PD-1 expressing T-cell subsets, two subsets had decreased expression of CD27 and CD28 when compared to controls. While the total number of PD-1-expressing T cells was not associated with FL patient outcome, we found that increased numbers of PD-1+ T cells exhibiting decreased CD27 and CD28 expression was associated with poorer patient survival. We found that T cells with decreased CD27 and CD28 expression tended to lose expression of other functional T cell markers, failed to proliferate when stimulated, and appeared terminally differentiated. Furthermore, we found that CD70+ lymphoma cells play an important role in downregulating expression of CD27 and CD28 on T cells. Taken together, our mass cytometry results have identified novel CD4+ memory T cell populations that are poorly functional and are associated with an inferior survival in FL.

 

 

Biography:

Bibigul Ilyassova has completed his PhD at the age of 32 years from  Kazakh Nat Medical University. She  is the Senior Researcher  and Associate professor  of  the Syzganov national Scientific Center of Surgery.  has published more than 15 papers in reputed journals

 

Abstract:

Cirrhosis in the outcome of chronic hepatitis D (CHD) is the most common cause of liver transplantation in Kazakhstan . The CHD is an immune-mediated disease The aim of the study was to determine the role of cytokines in the progression of liver fibrosis in chronic hepatitis D

A total of 105 patients with CHD and cirrhosis caused by HDV-infection were examined.  We uses ELISA tests and Fibroscan 502

The results showed the level of cytokines increase of TNF-α (P =0.009), IL-10 (P=0.002), depending on the stage of fibrosis. TNFα has a positive correlation with  ALT (r =0.358, P<0.0001), AST (r=0.452, P<0.0001) and negative with creatinine ​​(r=-0.396, P=0.002), urea (r=-0.280, P=0.019), platelets (r=-0.290, P=0.005); and  leukocytes (r=-0.342, P = 0.001). IL-10 has a positive  correlation with ALT  (r=0.256, P=0.004), AST (r=0.380, P<0.0001), bilirubin (r=0.194, P=0.037 ), and negative with albumin ​​(r=-0.586, P=0.005), creatinine (r=-0.389, P=0.003), urea (r=-0.267,P=0.026), platelets (r=-0.379, P<0.0001); and leukocytes (r=-0.382, P<0.0001). IL-17 has negative relationship with ALT and AST (r=-0.209, P=0.021 and r=-0.249, P=0.006).TGFβ1 has a positive relationship with ALT (r=0,263, P = 0,014), AST (r=0,263, P=0,004), albumin (r=0,600, P=0,004), total protein (r=0,296,P=0,027), TNFα (r=0,897, P<0.0001), IL-10 (r = 0.665, P <0.0001), and negative with IL-17 (r=-0.677, P <0.0001) and creatinine (r=-0.354, P=0.014).

Conclusion:    CHD  progression  is associated with the activity of the  of TNF-α and  IL-10, which have a negative relationship with IL-17 and positive with TGFβ1. The initiation of autoimmune inflammation occurs in the early stages fibrosis and  in cirrhosis occurs decrese of autoimmune inflammation